DNA

Part:BBa_K2961000:Design

Designed by: Muhammad Nahin Khan   Group: iGEM19_CMUQ   (2019-10-13)


CFTR Mutant Gene Segment (rs75389940_G)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

This CFTR gene segment was generated through CHOPCHOP by Harvard, and verified through BLAST and other genome browsing softwares. Note that this means there is a convenient PAM site near the gene on the genome that can be used by Cpf1 for targeting using gRNA.

References